proliferation marker ki67  (Elabscience Biotechnology)

Journal: Translational Cancer Research

Article Title: Obovatol induces apoptosis in breast cancer by downregulating the PI3K/Akt pathway

doi: 10.21037/tcr-2025-1-2627

Figure Lengend Snippet: Ob reduced the development of MDA-MB-231 cells in vivo . (A) The timetable for Ob-treated mice with xenograft tumors originating from MDA-MB-231 cells. (B-D) Effects of Ob treatment on tumor volume and weight. (E) Effects of Ob treatment on extending survival duration in mice. (F) Effects of Ob treatment on the body weight index of mice. (G) IHC assays were used to quantify the expression of PCNA, Ki67, Bcl-2, and Bax in tumor tissues. (H) Typical histological pictures of tumor slices stained with H&E. The tumor volume and body weight of the mice were checked every two days. *, P<0.05; **, P<0.01; ***, P<0.001 ( vs. control group). ADR, doxorubicin hydrochloride; Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2; Con, control; H&E, hematoxylin and eosin; IHC, immunohistochemistry; Ob, obovatol; PCNA, proliferating cell nuclear antigen.

Article Snippet: The following primary antibodies were used: proliferating cell nuclear antigen (PCNA; bs-2007R), Ki67 (bs-52455R), Bax (bs-52316R), and rabbit secondary antibody (bs-0295G-HRP) were obtained from Bioss (Beijing, China).

Techniques: In Vivo, Expressing, Staining, Control, Immunohistochemistry

Journal: bioRxiv

Article Title: Primary cilia promote resistance to EGFR tyrosine kinase inhibitor, osimertinib, in non–small cell lung cancer

doi: 10.64898/2026.03.03.709408

Figure Lengend Snippet: a, Luciferase-labeled human H1975-OR cells transduced with LacZ shRNA or IFT88 shRNA were injected into the left thorax of SCID mice. Mice were imaged for luminescence, administered 5 mg/kg osimertinib on the indicated days, and re-imaged near the experimental endpoint. b, The growth of the tumor was monitored through luminescence, and the average luminescence intensity at each time point was plotted. Total luminescence intensity (photon count) was calculated using live imaging software. Error bars indicate SEM. ** P < 0.01, two-way ANOVA followed by Tukey’s multiple comparisons test. c, (Top) Immunofluorescence was performed using a monoclonal Ki-67 antibody (green) and DAPI (blue) on cryo-sectioned lung tissues obtained from mice (n=3 per group) of the human lung tumor orthotopic model. Scale bar, 100 μm. (Bottom) H&E staining of serial sections of the related tissue. d, Immunofluorescence was performed using ARL13B (green) to mark cilia, ɣ-Tubulin (red) for centrioles, and DAPI (blue) for nuclei on cryo-sectioned lung tissues obtained from mice of the human lung tumor orthotopic model. Arrows indicate cilia. Scale bar, 10 μm. e, Quantification of d . Unpaired t-test.

Article Snippet: Frozen sections of the lung tissues harvested from the in vivo orthotopic lung cancer model were fixed in 4% paraformaldehyde) and stained for cilia markers ARL13B (Proteintech, 1:200), ɣ-Tubulin (Sigma, 1:200), and proliferation marker Ki67 (CST, Cat no. 12202S, 1:200) 24 hours after sectioning.

Techniques: Luciferase, Labeling, Transduction, shRNA, Injection, Imaging, Software, Immunofluorescence, Staining

Journal: bioRxiv

Article Title: Primary cilia promote resistance to EGFR tyrosine kinase inhibitor, osimertinib, in non–small cell lung cancer

doi: 10.64898/2026.03.03.709408

Figure Lengend Snippet: a , Human HCC827-OR cells transduced with LacZ shRNA or IFT88 shRNA were injected subcutaneously into the left flanks of SCID mice. Mice were administered 5 mg/kg osimertinib on the indicated days. Tumor volume was measured every 5 days with luminescence imaging. b , Images of tumors harvested from mice at the conclusion of the experiment. c , Tumor weight at the conclusion of the experiment. Unpaired t-test. d , Immunohistochemistry was performed using a monoclonal Ki-67 antibody (green) and DAPI (blue) on cryo-sectioned lung tissues (n = 5 mice in the LacZ shRNA group and n = 7 in the IFT88 shRNA group) obtained from subcutaneous human lung tumor model mice. e , Quantification of Ki-67 positive cells in d . Unpaired t-test. f , Immunohistochemistry was performed using ARL13B (green) to mark cilia, ɣ-tubulin (red) for centrioles, and DAPI (blue) for nuclei on cryo-sectioned lung tissues obtained from mice of the human lung tumor model. g , Quantification of f . Unpaired t-test.

Article Snippet: Frozen sections of the lung tissues harvested from the in vivo orthotopic lung cancer model were fixed in 4% paraformaldehyde) and stained for cilia markers ARL13B (Proteintech, 1:200), ɣ-Tubulin (Sigma, 1:200), and proliferation marker Ki67 (CST, Cat no. 12202S, 1:200) 24 hours after sectioning.

Techniques: Transduction, shRNA, Injection, Imaging, Immunohistochemistry